2025, Vol. 12, No. 4. - go to content...

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DOI: 10.15862/21ECOR425 (https://doi.org/10.15862/21ECOR425)

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Sakayan D.I., Khokhlachev N.S., Cheveleva A.N., Pinaev V.E. Selection and selection of strains capable of granule formation for intensification of the wastewater treatment process. Russian journal of resources, conservation and recycling. 2025; 12(4). Available at: https://resources.today/PDF/21ECOR425.pdf (in Russian). DOI: 10.15862/21ECOR425

Selection and selection of strains capable of granule formation for intensification of the wastewater treatment process

Sakayan Daniil Igorevich
Gazprom VNIIGAZ LLC, Moscow, Russia
E-mail: D_Sakayan@vniigaz.gazprom.ru
RSCI: https://elibrary.ru/author_profile.asp?id=1152014

Khokhlachev Nikolay Sergeevich
Gazprom VNIIGAZ LLC, Moscow, Russia
E-mail: N_Khokhlachev@vniigaz.gazprom.ru
RSCI: https://elibrary.ru/author_profile.asp?id=691378

Cheveleva Anna Nikolaevna
Gazprom VNIIGAZ LLC, Moscow, Russia
E-mail: A_Cheveleva@vniigaz.gazprom.ru
RSCI: https://elibrary.ru/author_profile.asp?id=81703

Pinaev Vladimir Evgenievich
Peoples’ Friendship University of Russia named after Patrice Lumumba, Moscow, Russia
Institute of Ecology
E-mail: pinaev_ve@pfur.ru

Abstract. Increasing the efficiency and compactness of wastewater treatment facilities is a pressing issue in the face of stricter environmental regulations and a shortage of space. One promising technology for process intensification is the use of granular aerobic sludge, which has good sedimentation properties and allows for the combined processes of nitrification, denitrification, and phosphorus removal in a single reactor. However, the widespread adoption of granular aerobic sludge is hampered by the long and unstable granule formation process. The aim of this study was to select and screen specific microbial strains capable of accelerating the formation of stable granules. To this end, the most resistant isolates were selected from a community of granular aerobic sludge formed over 90 days from activated sludge from the Stupino wastewater treatment plant using controlled oxidative stress (treatment with sublethal doses of hydrogen peroxide). Five strains were isolated and identified, two of which were Exophiala sp. (Isolate No. 2) and the bacterium Microbacterium paraoxydans (Isolate No. 3) demonstrated the ability to independently form granules. A bioaugmentation experiment showed that adding these cultures to the initial activated sludge reduced granule formation time to 11 and 14 days, respectively, which is 84–88 % faster than the control (90 days). These results confirm the high efficiency of using selected strains to intensify granular aerobic sludge technology and significantly reduce treatment plant startup time.

Keywords: biological wastewater treatment; granular aerobic activated sludge; strain selection; controlled oxidative stress

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